Kinetics of Hyaluronidase.

The determination of hyaluronidase activity as derived from its reaction kinetics.

The turbidimetric method, applied to the enzymatic hydrolysis of hyaluronate, was described by Kass and Seastone (1). Since then, various modifications have been suggested (2-4). A comprehensive discussion of the various assays and techniques has been published by Meyer (5). The purpose of the present work is to study and utilize the reaction kinetics involved in the hydrolysis of hyaluronate b...

Staphylococcal hyaluronidase.

Introduction Plasma Hyaluronidase (Hyal-1) Genomic Organization of the Hyaluronidase Sequences Hyaluronidase Genes on Chromosome 3p21.3 Hyaluronidase Genes on Chromosome 7q31.3 Implications for Clinical Medicine Hyaluronidases in Cancer

he has focused on the biology of hyaluronan and the hyaluronidases. He is also a board-certified Anatomic Pathologist and participates in the teaching and diagnostic services of the Department of Pathology. He is currently a visiting scientist in the laboratory of Dr. Robert Stern in San Francisco. In addition to hyaluronan and hyaluronidase, his interests include the process of aging and the b...

Cloning and expression of the enzymatic region of Streptococcal hyaluronidase

Objective(s): Streptococcus pyogenes produces extracellular hyaluronidase enzyme. This enzyme is directly associated with the spread of the organism during infection. The objective of the present study was to clone and express the nucleotide sequence of the enzyme which is involved in hyaluronidase enzymatic activity. Materials and Methods: The enzymatic region of hyaluronidase gene was detecte...

Colorimetric determination of hyaluronidase activity.

Turbidimetric methods for assay of hyaluronidase activity are based upon the measurement of the optical density of suspensions of substrateprotein combination in an acid environment (1). The reliability of this measurement depends upon careful control of the conditions under which turbidity develops (2), and even then the relation between substrate concentration and optical density is linear ov...